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KMID : 0617220030140010209
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Duksung Bulletin Phamaceutical Sciences
2003 Volume.14 No. 1 p.209 ~ p.211
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Sauchinone, a Lignan from Saururus chinenis, Inhibits Staurosporine-Induced Apoptosis in C6 Rat Glioma Cells
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Song, Hyun
Kim, Young Choong/Moon, Aree
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Abstract
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Neuronal apoptosis may contribute to pathologic neuronal loss in certain desease states such as neurodegenerative diseases. Staurosporine(ST), a nonselective protein kinase inhibitor, has been shown to induce apoptosis in a variety of cells including nerve cell lines. In this study, we investigated the neuroprotective effect of sauchinone, which is a unique lignan from Saururus chinensis, on ST-induced apoptosis in C6 rat glioma cells. Sauchinone attenuated ST-induced apoptosis of C6 glioma cells as evidenced by DNA fragmentation. We also provide evidence that the inhibitory effect of sauchinone on ST-induced apoptosis involves a dose-dependent upregulation of an antiapoptotic protein, Bcl-2, Mounting evidence shows that the activation of caspases, especially caspase-3, triggers the apoptotic process. The activity of caspase-3 of ST-pretreated cells was significantly decreased upon sauchinone treatment in a dose-dependent manner. Taken together, the data demonstrate that sauchinone protects C6 glioma cells from ST-induced apoptosis in a caspase-3 dependent manner. Our findings may be critical for developing a strategy to protect nerve cells from apoptosis, suggesting the potential development of sauchinone as a neuroprotective agent.
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